Recombinant:Article Title: The calcium-binding protein S100β regulates synaptic plasticity in the visual cortex
Article Snippet: .. *p < 0.05, **p < 0.01, ***p < 0.001 (Wilcoxon rank-signed test) 271 STAR★METHODSKEY RESOURCES TABLE 272 Jo urn al Pr e-p roo f 16 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-S100β Sigma-Aldrich Cat #S2532 RRID:AB_477499 Rabbit anti Kv4.2 Alomone Labs Cat #APC-023 RRID:AB_2040176 Chemicals, peptides, and recombinant proteins Potassium chloride Fisher Scientific #P217-500 Potassium phosphate monobasic Fisher Scientific #P285-500 Magnesium sulfate anhydrous Fisher Scientific #M65-500 Sodium bicarbonate Fisher Scientific #S233-500 Dextrose Fisher Scientific #D16-500 Calcium chloride dihydrate FisherScientific #C70-500 Sucrose Sigma-Aldrich #S9378 Sodium chloride Fisher Scientific #S671-3 Potassium D-gluconate Sigma-Aldrich #G4500-100G Magnesium chloride Sigma-Aldrich #68475-100ML-F HEPES Sigma-Aldrich #H3375-100G EGTA Sigma-Aldrich #E4378 Tris ATP salt Sigma-Aldrich #A9062 Tris GTP salt Sigma-Aldrich #G9002 1,2-Bis(2-aminophenoxy)-ethaneN,N,N’,N’-tetraacetic acid, tetrasodium salt hydrate Thermo Fisher Scientific #401650010 S100β Department of Biochemistry, Université de Montréal N/A 4-Aminopyridine Tocris Bioscience #0940 Suramin Tocris Bioscience #1472 L701,324 Tocris Bioscience #0907 DPCPX Tocris Bioscience #0439 SCH-58261 Tocris Bioscience #2270 Experimental models: Cell lines Mouse: C57BL/6J Bred in house after obtaining from The Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: B6Brd; B6N-Tyrc-Brd S100btm1a(EUCOMM)Wtsi/WtsiCnbc Wellcome Trust Sanger Institute RRID: MGI:5790617 Oligonucleotides S100b_47993_F: AGCTGGATCTTATGAGGGCG Invitrogen 10336022-521155 A01 CAS_R1_Term: TCGTGGTATCGTTATGCGCC Invitrogen 10336022-521155 A02 S100b_47993_R: CCAATCCCAGAGGTCTCCAG Invitrogen 10336022-521155 A03 LacZ_2_small_F: ATCACGACGCGCTGTATC Invitrogen 10336022-521155 A04 LacZ_2_small_R: ACATCGGGCAAATAATATCG Invitrogen 10336022-521155 A05 Software and algorithms Fluoview 2 Olympus #F10SWARS2 pClamp 8 Molecular Devices #6190-013 Clampfit 10 Molecular Devices https://www.moleculardevices.com/ products Jo urn al Pr e-p roo f 17 MultiClamp Molecular Devices https://www.moleculardevices.com/ products Axopatch Molecular Devices RRID:SCR_018866 SPSS 26-29 IBM SPSS Statistic RRID:SCR_002865 ImageJ National Institute of Health RRID:SCR_003070 Igor Pro WaveMetrics RRID:SCR_000325 273 EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 274 All experiments were conducted in accordance with the rules of the Canadian Institutes of Health Research and 275 were approved by the Animal Care and Use Committee of the University of Montreal (Protocol #23-207). ..
Software:Article Title: The calcium-binding protein S100β regulates synaptic plasticity in the visual cortex
Article Snippet: .. *p < 0.05, **p < 0.01, ***p < 0.001 (Wilcoxon rank-signed test) 271 STAR★METHODSKEY RESOURCES TABLE 272 Jo urn al Pr e-p roo f 16 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-S100β Sigma-Aldrich Cat #S2532 RRID:AB_477499 Rabbit anti Kv4.2 Alomone Labs Cat #APC-023 RRID:AB_2040176 Chemicals, peptides, and recombinant proteins Potassium chloride Fisher Scientific #P217-500 Potassium phosphate monobasic Fisher Scientific #P285-500 Magnesium sulfate anhydrous Fisher Scientific #M65-500 Sodium bicarbonate Fisher Scientific #S233-500 Dextrose Fisher Scientific #D16-500 Calcium chloride dihydrate FisherScientific #C70-500 Sucrose Sigma-Aldrich #S9378 Sodium chloride Fisher Scientific #S671-3 Potassium D-gluconate Sigma-Aldrich #G4500-100G Magnesium chloride Sigma-Aldrich #68475-100ML-F HEPES Sigma-Aldrich #H3375-100G EGTA Sigma-Aldrich #E4378 Tris ATP salt Sigma-Aldrich #A9062 Tris GTP salt Sigma-Aldrich #G9002 1,2-Bis(2-aminophenoxy)-ethaneN,N,N’,N’-tetraacetic acid, tetrasodium salt hydrate Thermo Fisher Scientific #401650010 S100β Department of Biochemistry, Université de Montréal N/A 4-Aminopyridine Tocris Bioscience #0940 Suramin Tocris Bioscience #1472 L701,324 Tocris Bioscience #0907 DPCPX Tocris Bioscience #0439 SCH-58261 Tocris Bioscience #2270 Experimental models: Cell lines Mouse: C57BL/6J Bred in house after obtaining from The Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: B6Brd; B6N-Tyrc-Brd S100btm1a(EUCOMM)Wtsi/WtsiCnbc Wellcome Trust Sanger Institute RRID: MGI:5790617 Oligonucleotides S100b_47993_F: AGCTGGATCTTATGAGGGCG Invitrogen 10336022-521155 A01 CAS_R1_Term: TCGTGGTATCGTTATGCGCC Invitrogen 10336022-521155 A02 S100b_47993_R: CCAATCCCAGAGGTCTCCAG Invitrogen 10336022-521155 A03 LacZ_2_small_F: ATCACGACGCGCTGTATC Invitrogen 10336022-521155 A04 LacZ_2_small_R: ACATCGGGCAAATAATATCG Invitrogen 10336022-521155 A05 Software and algorithms Fluoview 2 Olympus #F10SWARS2 pClamp 8 Molecular Devices #6190-013 Clampfit 10 Molecular Devices https://www.moleculardevices.com/ products Jo urn al Pr e-p roo f 17 MultiClamp Molecular Devices https://www.moleculardevices.com/ products Axopatch Molecular Devices RRID:SCR_018866 SPSS 26-29 IBM SPSS Statistic RRID:SCR_002865 ImageJ National Institute of Health RRID:SCR_003070 Igor Pro WaveMetrics RRID:SCR_000325 273 EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 274 All experiments were conducted in accordance with the rules of the Canadian Institutes of Health Research and 275 were approved by the Animal Care and Use Committee of the University of Montreal (Protocol #23-207). ..
|
Alomone Labs is a verified supplier 
Alomone Labs manufactures this product 